An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Control of Multicellular Development by the Physically Interacting Deneddylases DEN1/DenA and COP9 Signalosome.

Christmann M, Schmaler T, Gordon C, Huang X, Bayram O, Schinke J, Stumpf S, Dubiel W, Braus GH

Deneddylases remove the ubiquitin-like protein Nedd8 from modified proteins. An increased deneddylase activity has been associated with various human cancers. In contrast, we show here that a mutant strain of the model fungus Aspergillus nidulans deficient in two deneddylases is viable but can only grow as a filament and is highly impaired for multicellular development. The DEN1/DenA and the COP9 ... [more]

PLoS Genet. Feb. 01, 2013; 9(2);e1003275 [Pubmed: 23408908]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
COPS2 SENP8	Far Western Far Western An interaction is detected between a protein immobilized on a membrane and a purified protein probe.	Christmann M (2013)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

COPS2

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]signal transducer activity [NAS]

Gene Ontology Cellular Component

SENP8