An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Nuclear localization of Duplin, a beta-catenin-binding protein, is essential for its inhibitory activity on the Wnt signaling pathway.

Kobayashi M, Kishida S, Fukui A, Michiue T, Miyamoto Y, Okamoto T, Yoneda Y, Asashima M, Kikuchi A

Duplin binds to beta-catenin and inhibits the Wnt signaling pathway, thereby leading to repression of the beta-catenin-mediated transactivation and Xenopus axis formation. To find an additional function of Duplin, yeast two-hybrid screening was carried out. Importin alpha was isolated as a binding protein of Duplin. Importin alpha bound directly to basic amino acid clusters of Duplin. Although Duplin was present ... [more]

J. Biol. Chem. Feb. 22, 2002; 277(8);5816-22 [Pubmed: 11744694]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

CHD8

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [ISO]DNA binding [IDA, ISO]DNA helicase activity [ISO]DNA-dependent ATPase activity [ISO]armadillo repeat domain binding [ISO]beta-catenin binding [ISO]chromatin binding [ISO]histone binding [IDA, IPI]methylated histone binding [ISO]p53 binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

KPNA1