CHD8
Gene Ontology Biological Process
- ATP catabolic process [ISO]
- ATP-dependent chromatin remodeling [ISO]
- DNA duplex unwinding [ISO]
- brain development [ISO]
- canonical Wnt signaling pathway [ISO]
- digestive tract development [ISO]
- in utero embryonic development [IMP]
- negative regulation of Wnt signaling pathway [ISO]
- negative regulation of apoptotic process [IDA, IMP]
- negative regulation of canonical Wnt signaling pathway [ISO]
- negative regulation of fibroblast apoptotic process [IDA]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription, DNA-templated [IDA, ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of transcription from RNA polymerase III promoter [ISO]
- positive regulation of transcription, DNA-templated [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
KPNA2
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cell [IDA]
- cytoplasm [ISO]
- intracellular [IDA]
- membrane [ISO]
- nucleoplasm [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Nuclear localization of Duplin, a beta-catenin-binding protein, is essential for its inhibitory activity on the Wnt signaling pathway.
Duplin binds to beta-catenin and inhibits the Wnt signaling pathway, thereby leading to repression of the beta-catenin-mediated transactivation and Xenopus axis formation. To find an additional function of Duplin, yeast two-hybrid screening was carried out. Importin alpha was isolated as a binding protein of Duplin. Importin alpha bound directly to basic amino acid clusters of Duplin. Although Duplin was present ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CHD8 KPNA2 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID