An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Doublecortin-like kinase functions with doublecortin to mediate fiber tract decussation and neuronal migration.

Koizumi H, Tanaka T, Gleeson JG

The potential role of doublecortin (Dcx), encoding a microtubule-associated protein, in brain development has remained controversial. Humans with mutations show profound alterations in cortical lamination, whereas in mouse, RNAi-mediated knockdown but not germline knockout shows abnormal positioning of cortical neurons. Here, we report that the doublecortin-like kinase (Dclk) gene functions in a partially redundant pathway with Dcx in the formation ... [more]

Neuron Jan. 05, 2006; 49(1);55-66 [Pubmed: 16387639]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

DCX

Gene Ontology Biological Process

Gene Ontology Molecular Function

microtubule binding [ISO]protein binding [IPI]protein kinase binding [ISO]

Gene Ontology Cellular Component

DCLK1