BAIT

MKK4

ARABIDOPSIS THALIANA MITOGEN-ACTIVATED PROTEIN KINASE KINASE 4, ATMEK4, ATMKK4, F19C24.26, F19C24_26, MAP KINASE KINASE 4, mitogen-activated protein kinase kinase 4, AT1G51660
mitogen-activated protein kinase kinase 4
Arabidopsis thaliana (Columbia)
PREY

MYBR1

ARABIDOPSIS THALIANA MYB DOMAIN PROTEIN 44, ATMYB44, ATMYBR1, MYB44, myb domain protein r1, AT5G67300
transcription factor MYB44
Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Tight Interconnection and Multi-Level Control of Arabidopsis MYB44 in MAPK Cascade Signalling.

Persak H, Pitzschke A

Abiotic stress poses a huge, ever-increasing problem to plants and agriculture. The dissection of signalling pathways mediating stress tolerance is a prerequisite to develop more resistant plant species. Mitogen-activated protein kinase (MAPK) cascades are universal signalling modules. In Arabidopsis, the MAPK MPK3 and its upstream regulator MAPK kinase MKK4 initiate the adaptation response to numerous abiotic and biotic stresses. Yet, ... [more]

PLoS ONE Feb. 26, 2013; 8(2);e57547 [Pubmed: 23437396]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MKK4 MYBR1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID