An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A proteomic snapshot of the human heat shock protein 90 interactome.

Falsone SF, Gesslbauer B, Tirk F, Piccinini AM, Kungl AJ

Heat shock protein 90 (Hsp90) is a molecular chaperone which modulates several signalling pathways within a cell. By applying co-immunoprecipitation with endogeneous Hsp90, we were able to identify 39 novel protein interaction partners of this chaperone in human embryonic kidney cells (HEK293). Interestingly, levels of DNA-activated protein kinase catalytic subunit, an Hsp90 interaction partner found in this study, were found ... [more]

FEBS Lett. Nov. 21, 2005; 579(28);6350-4 [Pubmed: 16263121]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
HSP90AB1 HSPH1	Cross-Linking-MS (XL-MS) Cross-Linking-MS (XL-MS) An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).	Wheat A (2021)	High	-	BioGRID	3678518

Curated By

BioGRID

Interaction Summary

HSP90AB1

Gene Ontology Biological Process

Gene Ontology Molecular Function

MHC class II protein complex binding [IDA]TPR domain binding [ISS]double-stranded RNA binding [IDA]kinase binding [IPI]nitric-oxide synthase regulator activity [ISS]poly(A) RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

HSPH1