BAIT

NEO1

putative aminophospholipid-translocating P4-type ATPase NEO1, L000004112, YIL048W
Putative aminophospholipid translocase (flippase); involved in endocytosis, vacuolar biogenesis and Golgi to ER vesicle-mediated transport; localizes to endosomes and the Golgi apparatus
Saccharomyces cerevisiae (S288c)
PREY

APL6

YKS5, L000003034, YGR261C
Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools
GO Process (2)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Publication

Yeast and human Ysl2p/hMon2 interact with Gga adaptors and mediate their subcellular distribution.

Singer-Krueger B, Lasic M, Buerger AM, Hausser A, Pipkorn R, Wang Y

The Gga proteins represent a family of ubiquitously expressed clathrin adaptors engaged in vesicle budding at the tubular endosomal network/trans Golgi network. Their membrane recruitment is commonly thought to involve interactions with Arf and signals in cargo through the so-called VHS domain. For yeast Gga proteins, however, partners binding to its VHS domain have remained elusive and Gga localization does ... [more]

EMBO J. May. 21, 2008; 27(10);1423-35 [Pubmed: 18418388]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: heat sensitivity (APO:0000147) [heat sensitivity (APO:0000147)]

Additional Notes

  • The neo1-69 temperature-sensitive mutant can be suppressed by gene deletions of adaptor proteins.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NEO1 APL6
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1568BioGRID
388078

Curated By

  • BioGRID