BAIT

ULP1

NIB1, SUMO protease ULP1, S000029323, L000001249, YPL020C
Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions
GO Process (2)
GO Function (2)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

GRX3

monothiol glutaredoxin GRX3, S000007444, YDR098C
Glutathione-dependent oxidoreductase; hydroperoxide and superoxide-radical responsive; monothiol glutaredoxin subfamily member along with Grx4p and Grx5p; protects cells from oxidative damage; with Grx4p, binds to Aft1p in iron-replete conditions, promoting its dissociation from promoters; evidence exists indicating that the translation start site is not Met1 as currently annotated, but rather Met36; GRX3 has a paralog, GRX4, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Genome-wide bimolecular fluorescence complementation analysis of SUMO interactome in yeast.

Sung MK, Lim G, Yi DG, Chang YJ, Yang EB, Lee K, Huh WK

The definition of protein-protein interactions (PPIs) in the natural cellular context is essential for properly understanding various biological processes. So far, however, most large-scale PPI analyses have not been performed in the natural cellular context. Here, we describe the construction of a Saccharomyces cerevisiae fusion library in which each endogenous gene is C-terminally tagged with the N-terminal fragment of Venus ... [more]

Genome Res. Feb. 12, 2013; 0(0); [Pubmed: 23403034]

Throughput

  • High Throughput

Additional Notes

  • bimolecular fluorescence complementation (BiFC) assay; hit protein contains VC-tagged Smt3p; signal intensity of cells overexpressing bait protein is compared to that of control cells without overexpression to detect desumoylation substrates of bait protein

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ULP1 GRX3
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
GRX3 ULP1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2693BioGRID
2034116

Curated By

  • BioGRID