BAIT

GAD1

glutamate decarboxylase GAD1, S000007509, YMR250W
Glutamate decarboxylase; converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress
GO Process (2)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

UGA2

UGA5, succinate-semialdehyde dehydrogenase (NAD(P)(+)), S000007516, YBR006W
Succinate semialdehyde dehydrogenase; involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm
GO Process (3)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

GABA shunt mediates thermotolerance in Saccharomyces cerevisiae by reducing reactive oxygen production.

Cao J, Barbosa JM, Singh NK, Locy RD

The GABA shunt pathway involves three enzymes: glutamate decarboxylase (GAD), GABA aminotransferase (GAT), and succinate semialdehyde dehydrogenase (SSADH). These enzymes act in concert to convert glutamate (α-ketoglutarate) to succinate. Deletion mutations in each of these genes in Saccharomyces cerevisiae resulted in growth defects at 45°C. Double and triple mutation constructs were compared for thermotolerance with the wild type and single ... [more]

Yeast Feb. 28, 2013; 0(0); [Pubmed: 23447388]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: heat sensitivity (APO:0000147)
  • phenotype: inviable (APO:0000112)

Additional Notes

  • gad1 uga1 uga2 triple mutant has increased heat sensitivity to stress at 45 and 50 degrees

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GAD1 UGA2
Phenotypic Enhancement
Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
825244
GAD1 UGA2
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Low-BioGRID
825238

Curated By

  • BioGRID