BAIT

NME2

NDKB, nm23-2, p18-12d
NME/NM23 nucleoside diphosphate kinase 2
GO Process (20)
GO Function (6)
GO Component (12)
Rattus norvegicus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Interactions of phocein with nucleoside-diphosphate kinase, Eps15, and Dynamin I.

Baillat G, Gaillard S, Castets F, Monneron A

Phocein, an intracellular protein interacting with striatin, bears a few homologies with the sigma-subunits of clathrin adaptor proteins (Baillat, G., Moqrich, A., Castets, F., Baude, A., Bailly, Y., Benmerah, A., and Monneron, A. (2001) Mol. Biol. Cell 12, 663-673). Using phocein as a bait in a yeast two-hybrid screen, we identified two novel interacting proteins, nucleoside-diphosphate kinase (NDPK) and Eps15. ... [more]

J. Biol. Chem. May. 24, 2002; 277(21);18961-6 [Pubmed: 11872741]

Throughput

  • Low Throughput

Curated By

  • BioGRID