An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Identification of a novel muscle A-type lamin-interacting protein (MLIP).

Ahmady E, Deeke SA, Rabaa S, Kouri L, Kenney L, Stewart AF, Burgon PG

Mutations in the A-type lamin (LMNA) gene are associated with age-associated degenerative disorders of mesenchymal tissues, such as dilated cardiomyopathy, Emery-Dreifuss muscular dystrophy, and limb-girdle muscular dystrophy. The molecular mechanisms that connect mutations in LMNA with different human diseases are poorly understood. Here, we report the identification of a Muscle-enriched A-type Lamin-interacting Protein, MLIP (C6orf142 and 2310046A06rik), a unique single ... [more]

J. Biol. Chem. Jun. 03, 2011; 286(22);19702-13 [Pubmed: 21498514]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

MLIP

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription [IGI]

Gene Ontology Cellular Component

LMNA