BAIT
FN1
CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF
fibronectin 1
GO Process (12)
GO Function (5)
GO Component (8)
Gene Ontology Biological Process
- blood coagulation [TAS]
- cell adhesion [NAS]
- endodermal cell differentiation [IDA]
- extracellular matrix disassembly [TAS]
- extracellular matrix organization [TAS]
- integrin activation [IMP]
- leukocyte migration [TAS]
- peptide cross-linking [IDA]
- platelet activation [TAS]
- platelet degranulation [TAS]
- response to wounding [NAS]
- substrate adhesion-dependent cell spreading [IDA, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ARF6
ADP-ribosylation factor 6
GO Process (14)
GO Function (4)
GO Component (10)
Gene Ontology Biological Process
- GTP catabolic process [TAS]
- cell adhesion [TAS]
- cellular component movement [TAS]
- cortical actin cytoskeleton organization [IMP]
- negative regulation of receptor-mediated endocytosis [TAS]
- positive regulation of actin filament polymerization [IMP]
- positive regulation of establishment of protein localization to plasma membrane [ISS]
- protein localization to cell surface [ISS]
- protein localization to endosome [IMP]
- regulation of Rac protein signal transduction [IDA]
- regulation of dendritic spine development [ISS]
- regulation of filopodium assembly [IDA]
- ruffle organization [IDA]
- vesicle-mediated transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Proteomic analysis of integrin-associated complexes identifies RCC2 as a dual regulator of Rac1 and Arf6.
The binding of integrin adhesion receptors to their extracellular matrix ligands controls cell morphology, movement, survival, and differentiation in various developmental, homeostatic, and disease processes. Here, we report a methodology to isolate complexes associated with integrin adhesion receptors, which, like other receptor-associated signaling complexes, have been refractory to proteomic analysis. Quantitative, comparative analyses of the proteomes of two receptor-ligand pairs, ... [more]
Sci Signal Sep. 10, 2009; 2(87);ra51 [Pubmed: 19738201]
Throughput
- High Throughput
Ontology Terms
- k-562 cell (BTO:0000664) [chronic myeloid leukemia (DOID:8552)]
Additional Notes
- exogenous expression of bait
Curated By
- BioGRID