Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Proteomic analysis of integrin-associated complexes identifies RCC2 as a dual regulator of Rac1 and Arf6.

Humphries JD, Byron A, Bass MD, Craig SE, Pinney JW, Knight D, Humphries MJ

The binding of integrin adhesion receptors to their extracellular matrix ligands controls cell morphology, movement, survival, and differentiation in various developmental, homeostatic, and disease processes. Here, we report a methodology to isolate complexes associated with integrin adhesion receptors, which, like other receptor-associated signaling complexes, have been refractory to proteomic analysis. Quantitative, comparative analyses of the proteomes of two receptor-ligand pairs, ... [more]

Sci Signal Sep. 10, 2009; 2(87);ra51 [Pubmed: 19738201]

Throughput

  • High Throughput

Ontology Terms

  • cell line: k-562 cell (BTO:0000664) [chronic myeloid leukemia (DOID:8552)]

Additional Notes

  • exogenous expression of bait

Curated By

  • BioGRID