AMPH
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CLTC
Gene Ontology Biological Process
- Golgi organization [IMP]
- mitotic nuclear division [IMP, ISO]
- negative regulation of hyaluronan biosynthetic process [ISO, ISS]
- negative regulation of protein localization to plasma membrane [ISO]
- osteoblast differentiation [ISO]
- receptor internalization [ISO]
- receptor-mediated endocytosis [IMP, ISO]
- transferrin transport [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- T-tubule [IDA]
- clathrin coat [IDA, TAS]
- clathrin complex [ISO]
- clathrin-coated endocytic vesicle membrane [TAS]
- clathrin-coated vesicle [ISO]
- cytoplasm [ISO]
- extracellular vesicular exosome [ISO]
- focal adhesion [ISO]
- intracellular membrane-bounded organelle [ISO]
- membrane [ISO]
- membrane coat [ISO]
- mitochondrion [ISO]
- myelin sheath [ISO]
- protein complex [ISO]
- sarcolemma [IDA]
- spindle [IDA, ISO]
- terminal bouton [IDA]
- vesicle [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The NECAP PHear domain increases clathrin accessory protein binding potential.
AP-2 is a key regulator of the endocytic protein machinery driving clathrin-coated vesicle (CCV) formation. One critical function, mediated primarily by the AP-2 alpha-ear, is the recruitment of accessory proteins. NECAPs are alpha-ear-binding proteins that enrich on CCVs. Here, we have solved the structure of the conserved N-terminal region of NECAP 1, revealing a unique module in the pleckstrin homology ... [more]
Throughput
- Low Throughput
Additional Notes
- figure 3.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| AMPH CLTC | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID