BAIT

GABARAPL2

ATG8, ATG8C, GATE-16, GATE16, GEF-2, GEF2
GABA(A) receptor-associated protein-like 2
Autophagy Project
Homo sapiens
PREY

BNIP3L

BNIP3a, NIX
BCL2/adenovirus E1B 19kDa interacting protein 3-like
Autophagy Project
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Nix is a selective autophagy receptor for mitochondrial clearance.

Novak I, Kirkin V, McEwan DG, Zhang J, Wild P, Rozenknop A, Rogov V, Loehr F, Popovic D, Occhipinti A, Reichert AS, Terzic J, Doetsch V, Ney PA, Dikic I

Autophagy is the cellular homeostatic pathway that delivers large cytosolic materials for degradation in the lysosome. Recent evidence indicates that autophagy mediates selective removal of protein aggregates, organelles and microbes in cells. Yet, the specificity in targeting a particular substrate to the autophagy pathway remains poorly understood. Here, we show that the mitochondrial protein Nix is a selective autophagy receptor ... [more]

EMBO Rep. Jan. 01, 2010; 11(1);45-51 [Pubmed: 20010802]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GABARAPL2 BNIP3L
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
2695819

Curated By

  • BioGRID