BAIT
HSP90AA1
EL52, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP-2, LAP2
heat shock protein 90kDa alpha (cytosolic), class A member 1
GO Process (16)
GO Function (10)
GO Component (10)
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- G2/M transition of mitotic cell cycle [TAS]
- axon guidance [TAS]
- chaperone-mediated protein complex assembly [IDA]
- innate immune response [TAS]
- mitochondrial transport [TAS]
- mitotic cell cycle [TAS]
- nitric oxide metabolic process [TAS]
- positive regulation of nitric oxide biosynthetic process [ISS]
- protein import into mitochondrial outer membrane [IDA]
- protein refolding [TAS]
- regulation of nitric-oxide synthase activity [TAS]
- response to unfolded protein [NAS]
- signal transduction [NAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
FOXM1
FKHL16, FOXM1B, HFH-11, HFH11, HNF-3, INS-1, MPHOSPH2, MPP-2, MPP2, PIG29, TGT3, TRIDENT
forkhead box M1
GO Process (20)
GO Function (5)
GO Component (2)
Gene Ontology Biological Process
- DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator [IMP]
- G2/M transition of mitotic cell cycle [IDA, TAS]
- cell cycle [TAS]
- mitotic cell cycle [TAS]
- negative regulation of cell aging [IMP]
- negative regulation of stress-activated MAPK cascade [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription, DNA-templated [IMP]
- positive regulation of cell proliferation [IMP]
- positive regulation of double-strand break repair [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of transcription, DNA-templated [IMP]
- regulation of Ras protein signal transduction [IMP]
- regulation of cell cycle [TAS]
- regulation of cell cycle arrest [IMP]
- regulation of cell growth [TAS]
- regulation of cell proliferation [NAS]
- regulation of reactive oxygen species metabolic process [IMP]
- regulation of transcription, DNA-templated [IDA, TAS]
- transcription from RNA polymerase II promoter [IBA, IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cytoplasm [IDA]
- nucleoplasm [IDA, TAS]
Homo sapiens
Affinity Capture-Luminescence
An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.
Publication
Quantitative analysis of HSP90-client interactions reveals principles of substrate recognition.
HSP90 is a molecular chaperone that associates with numerous substrate proteins called clients. It plays many important roles in human biology and medicine, but determinants of client recognition by HSP90 have remained frustratingly elusive. We systematically and quantitatively surveyed most human kinases, transcription factors, and E3 ligases for interaction with HSP90 and its cochaperone CDC37. Unexpectedly, many more kinases than ... [more]
Cell Aug. 31, 2012; 150(5);987-1001 [Pubmed: 22939624]
Throughput
- Low Throughput
Curated By
- BioGRID