BAIT

CDH1

Arc-1, CD324, CDHE, ECAD, LCAM, UVO
cadherin 1, type 1, E-cadherin (epithelial)
GO Process (17)
GO Function (7)
GO Component (16)
Homo sapiens
PREY

NIPSNAP1

nipsnap homolog 1 (C. elegans)
GO Process (0)
GO Function (0)
GO Component (1)

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Biosensor-based micro-affinity purification for the proteomic analysis of protein complexes.

Catimel B, Rothacker J, Catimel J, Faux M, Ross J, Connolly L, Clippingdale A, Burgess AW, Nice E

A biosensor-based micro-affinity purification method to recover protein binding partners and their complexes for down stream proteomics analysis has been developed using the BIAcore 3000 fitted with a prototype Surface Prep Unit (SPU). The recombinant GST-intracellular domain of E-cadherin or the recombinant GST-beta-catenin binding domain of Adenomatous Polyposis Coli (APC) were immobilized onto the SPU and used to affinity purify ... [more]

J. Proteome Res. Oct. 11, 2005; 4(5);1646-56 [Pubmed: 16212417]

Throughput

  • Low Throughput

Curated By

  • BioGRID