BAIT

PXN

hCG_1778014
paxillin
Homo sapiens
PREY

RNF-5

CELE_C16C10.7, C16C10.7
rnf-5 encodes a homolog of mammalian RNF5, an E3 ubiquitin ligase; in C. elegans, RNF-5 functions as a ubiquitin ligase that regulates activity of the UNC-95 LIM domain protein that is essential for proper formation of muscle attachment structures; RNF-5 is also required for distal tip cell migration (DTC) and genetic interactions suggest that RNF-5 functions at least partially in parallel with the coiled-coil domain protein CCDC-55 and the E3 ubiquitin ligase RNF-121 to regulate DTC migration; RNF-5 localizes to striated muscle dense bodies, cell junctions, and to the nucleus.
Caenorhabditis elegans

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

RNF5, a RING finger protein that regulates cell motility by targeting paxillin ubiquitination and altered localization.

Didier C, Broday L, Bhoumik A, Israeli S, Takahashi S, Nakayama K, Thomas SM, Turner CE, Henderson S, Sabe H, Ronai Z

RNF5 is a RING finger protein found to be important in the growth and development of Caenorhabditis elegans. The search for RNF5-associated proteins via a yeast two-hybrid screen identified a LIM-containing protein in C. elegans which shows homology with human paxillin. Here we demonstrate that the human homologue of RNF5 associates with the amino-terminal domain of paxillin, resulting in its ... [more]

Mol. Cell. Biol. Aug. 01, 2003; 23(15);5331-45 [Pubmed: 12861019]

Throughput

  • Low Throughput

Additional Notes

  • figure 2.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RNF-5 PXN
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
831306

Curated By

  • BioGRID