BAIT

NAC6

ANAC092, ATNAC2, ATNAC6, Arabidopsis NAC domain containing protein 92, MIJ24.11, MIJ24_11, NAC domain containing protein 2, NAC domain containing protein 6, NAC2, ORE1, ORESARA 1, AT5G39610
NAC-domain transcription factor
Arabidopsis thaliana (Columbia)
PREY

GLK2

ATGLK2, GBF'S PRO-RICH REGION-INTERACTING FACTOR 2, GOLDEN2-like 2, GPRI2, MLN1.11, MLN1_11, AT5G44190
transcription activator GLK2
Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

ORE1 balances leaf senescence against maintenance by antagonizing G2-like-mediated transcription.

Rauf M, Arif M, Dortay H, Matallana-Ramirez LP, Waters MT, Gil Nam H, Lim PO, Mueller-Roeber B, Balazadeh S

Leaf senescence is a key physiological process in all plants. Its onset is tightly controlled by transcription factors, of which NAC factor ORE1 (ANAC092) is crucial in Arabidopsis thaliana. Enhanced expression of ORE1 triggers early senescence by controlling a downstream gene network that includes various senescence-associated genes. Here, we report that unexpectedly ORE1 interacts with the G2-like transcription factors GLK1 ... [more]

EMBO Rep. Mar. 05, 2013; 0(0); [Pubmed: 23459204]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NAC6 GLK2
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Low-BioGRID
-
NAC6 GLK2
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID