BAIT

SMURF2

2810411E22Rik, AI558114, AI649275, RP23-170A23.3

SMAD specific E3 ubiquitin protein ligase 2

GO Process (5)

GO Function (5)

GO Component (3)

Gene Ontology Biological Process

protein ubiquitination [ISO]

protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IBA]

transforming growth factor beta receptor signaling pathway [IBA]

ubiquitin-dependent SMAD protein catabolic process [ISO]

ubiquitin-dependent protein catabolic process [ISO]

Gene Ontology Molecular Function

SMAD binding [ISO]
identical protein binding [ISO]
protein binding [IPI]
transforming growth factor beta receptor binding [ISO]
ubiquitin-protein transferase activity [IBA, ISO]

Gene Ontology Cellular Component

cytoplasm [IBA]

ubiquitin ligase complex [ISO]

CRISPR Database MGI Alliance of Genome Resources VEGA Entrez Gene RefSeq UniprotKB Ensembl

Mus musculus

PREY

TRP53BP1

53BP1, Tp53bp1, m53BP1, p53BP1, RP23-437L13.2

transformation related protein 53 binding protein 1

GO Process (5)

GO Function (9)

GO Component (6)

Gene Ontology Biological Process

DNA repair [IDA]

cellular response to DNA damage stimulus [ISO]

positive regulation of transcription from RNA polymerase II promoter [ISO]

regulation of transcription, DNA-templated [TAS]

transcription from RNA polymerase II promoter [ISO]

Gene Ontology Molecular Function

RNA polymerase II activating transcription factor binding [ISO]
RNA polymerase II transcription cofactor activity [ISO]
damaged DNA binding [IDA]
methylated histone binding [ISO]
p53 binding [ISO]
protein binding [IPI]
sequence-specific DNA binding [IDA]
telomeric DNA binding [IDA]
transcription factor binding [TAS]

Gene Ontology Cellular Component

chromosome, telomeric region [ISO]

cytoplasm [ISO]

kinetochore [IDA]

nucleoplasm [ISO]

nucleus [IDA, ISO]

replication fork [IDA]

CRISPR Database VEGA MGI Alliance of Genome Resources Entrez Gene RefSeq UniprotKB Ensembl

Mus musculus

Affinity Capture-Luminescence

An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.

Publication

Regulation of planar cell polarity by Smurf ubiquitin ligases.

Narimatsu M, Bose R, Pye M, Zhang L, Miller B, Ching P, Sakuma R, Luga V, Roncari L, Attisano L, Wrana JL

Planar cell polarity (PCP) is critical for morphogenesis in metazoans. PCP in vertebrates regulates stereocilia alignment in neurosensory cells of the cochlea and closure of the neural tube through convergence and extension movements (CE). Noncanonical Wnt morphogens regulate PCP and CE in vertebrates, but the molecular mechanisms remain unclear. Smurfs are ubiquitin ligases that regulate signaling, cell polarity and motility ... [more]

Cell Apr. 17, 2009; 137(2);295-307 [Pubmed: 19379695]

Throughput

Low Throughput

Additional Notes

figure 3.

Curated By

BioGRID