BAIT

NPHP4

4930564O18Rik, nmf192, RP23-352D7.1

nephronophthisis 4 (juvenile) homolog (human)

GO Process (4)

GO Function (1)

GO Component (6)

Gene Ontology Biological Process

photoreceptor cell maintenance [IMP]

photoreceptor cell outer segment organization [IMP]

retina development in camera-type eye [IMP]

sperm motility [IMP]

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

cell-cell junction [ISO]

centrosome [ISO]

ciliary base [IDA]

ciliary transition zone [IDA]

photoreceptor connecting cilium [IDA]

ribbon synapse [IDA]

CRISPR Database VEGA MGI Alliance of Genome Resources Entrez Gene RefSeq UniprotKB Ensembl

Mus musculus

PREY

HSPB1

27kDa, Hsp25

heat shock protein 1

GO Process (15)

GO Function (6)

GO Component (10)

Gene Ontology Biological Process

cellular response to vascular endothelial growth factor stimulus [ISO]

intracellular signal transduction [ISO]

negative regulation of apoptotic signaling pathway [IDA]

negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway [IDA]

negative regulation of protein kinase activity [IDA]

negative regulation of protein serine/threonine kinase activity [IDA]

platelet aggregation [ISO]

positive regulation of angiogenesis [ISO]

positive regulation of blood vessel endothelial cell migration [ISO]

positive regulation of endothelial cell chemotaxis [ISO]

positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway [ISO]

positive regulation of interleukin-1 beta production [IDA]

positive regulation of tumor necrosis factor biosynthetic process [IDA]

regulation of I-kappaB kinase/NF-kappaB signaling [IDA]

response to unfolded protein [NAS]

Gene Ontology Molecular Function

identical protein binding [ISO]
poly(A) RNA binding [ISO]
protein kinase C binding [IDA]
protein kinase C inhibitor activity [IDA]
protein kinase binding [ISO]
ubiquitin binding [ISO]

Gene Ontology Cellular Component

contractile fiber [IDA]

cytoplasm [IDA, ISO]

extracellular space [ISO]

extracellular vesicular exosome [ISO]

focal adhesion [ISO]

intracellular [IDA]

nucleus [IDA, ISO]

plasma membrane [IDA]

proteasome complex [ISO]

CRISPR Database VEGA MGI Alliance of Genome Resources Entrez Gene RefSeq UniprotKB Ensembl

Mus musculus

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Mapping the NPHP-JBTS-MKS protein network reveals ciliopathy disease genes and pathways.

Sang L, Miller JJ, Corbit KC, Giles RH, Brauer MJ, Otto EA, Baye LM, Wen X, Scales SJ, Kwong M, Huntzicker EG, Sfakianos MK, Sandoval W, Bazan JF, Kulkarni P, Garcia-Gonzalo FR, Seol AD, O'Toole JF, Held S, Reutter HM, Lane WS, Rafiq MA, Noor A, Ansar M, Devi AR, Sheffield VC, Slusarski DC, Vincent JB, Doherty DA, Hildebrandt F, Reiter JF, Jackson PK

Nephronophthisis (NPHP), Joubert (JBTS), and Meckel-Gruber (MKS) syndromes are autosomal-recessive ciliopathies presenting with cystic kidneys, retinal degeneration, and cerebellar/neural tube malformation. Whether defects in kidney, retinal, or neural disease primarily involve ciliary, Hedgehog, or cell polarity pathways remains unclear. Using high-confidence proteomics, we identified 850 interactors copurifying with nine NPHP/JBTS/MKS proteins and discovered three connected modules: "NPHP1-4-8" functioning at the ... [more]

Cell May. 13, 2011; 145(4);513-28 [Pubmed: 21565611]

Throughput

High Throughput

Curated By

BioGRID