PEX14
Gene Ontology Biological Process
- microtubule anchoring [IDA]
- negative regulation of protein binding [IDA]
- negative regulation of protein homotetramerization [IDA]
- negative regulation of sequence-specific DNA binding transcription factor activity [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- peroxisome organization [IGI, ISS]
- peroxisome transport along microtubule [IDA]
- protein complex assembly [IDA]
- protein homooligomerization [IDA]
- protein import into peroxisome matrix [IMP]
- protein import into peroxisome matrix, substrate release [IDA]
- protein import into peroxisome matrix, translocation [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PEX19
Gene Ontology Biological Process
- chaperone-mediated protein folding [IDA]
- chaperone-mediated protein transport [IDA]
- establishment of protein localization to peroxisome [IMP]
- negative regulation of lipid binding [IDA]
- peroxisome fission [IMP]
- peroxisome membrane biogenesis [IDA]
- peroxisome organization [IMP, NAS]
- protein import into peroxisome membrane [IDA]
- protein stabilization [IDA]
- protein targeting to peroxisome [IDA, IMP]
- transmembrane transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Co-purification
An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.
Publication
PEX14 is required for microtubule-based peroxisome motility in human cells.
We have established a procedure for isolating native peroxisomal membrane protein complexes from cultured human cells. Protein-A-tagged peroxin 14 (PEX14), a central component of the peroxisomal protein translocation machinery was genomically expressed in Flp-In-293 cells and purified from digitonin-solubilized membranes. Size-exclusion chromatography revealed the existence of distinct multimeric PEX14 assemblies at the peroxisomal membrane. Using mass spectrometric analysis, almost all ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PEX14 PEX19 | Affinity Capture-Luminescence Affinity Capture-Luminescence An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag. | Low | - | BioGRID | 2751939 | |
| PEX14 PEX19 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | Low | - | BioGRID | - | |
| PEX19 PEX14 | FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins. | High | - | BioGRID | 2598458 | |
| PEX14 PEX19 | FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins. | High | - | BioGRID | 2598373 | |
| PEX14 PEX19 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | 57.5 | BioGRID | 2999488 | |
| PEX19 PEX14 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| PEX14 PEX19 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| PEX19 PEX14 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID