BAIT
PEX14
NAPP2, PBD13A, Pex14p, dJ734G22.2
peroxisomal biogenesis factor 14
GO Process (12)
GO Function (6)
GO Component (9)
Gene Ontology Biological Process
- microtubule anchoring [IDA]
- negative regulation of protein binding [IDA]
- negative regulation of protein homotetramerization [IDA]
- negative regulation of sequence-specific DNA binding transcription factor activity [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- peroxisome organization [IGI, ISS]
- peroxisome transport along microtubule [IDA]
- protein complex assembly [IDA]
- protein homooligomerization [IDA]
- protein import into peroxisome matrix [IMP]
- protein import into peroxisome matrix, substrate release [IDA]
- protein import into peroxisome matrix, translocation [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
IQGAP1
HUMORFA01, SAR1, p195
IQ motif containing GTPase activating protein 1
GO Process (13)
GO Function (9)
GO Component (17)
Gene Ontology Biological Process
- cellular response to calcium ion [IDA]
- cellular response to epidermal growth factor stimulus [IMP]
- energy reserve metabolic process [TAS]
- epidermal growth factor receptor signaling pathway [IMP]
- glomerular visceral epithelial cell development [ISS]
- negative regulation of catalytic activity [TAS]
- neuron projection extension [IMP]
- positive regulation of GTPase activity [TAS]
- positive regulation of protein kinase activity [IMP]
- positive regulation of protein serine/threonine kinase activity [IDA]
- regulation of insulin secretion [TAS]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function- GTPase activator activity [TAS]
- GTPase inhibitor activity [TAS]
- calcium ion binding [TAS]
- calmodulin binding [IPI]
- phosphatidylinositol-3,4,5-trisphosphate binding [IDA]
- protein binding [IPI]
- protein kinase binding [IPI]
- protein phosphatase binding [IPI]
- protein serine/threonine kinase activator activity [IDA]
- GTPase activator activity [TAS]
- GTPase inhibitor activity [TAS]
- calcium ion binding [TAS]
- calmodulin binding [IPI]
- phosphatidylinositol-3,4,5-trisphosphate binding [IDA]
- protein binding [IPI]
- protein kinase binding [IPI]
- protein phosphatase binding [IPI]
- protein serine/threonine kinase activator activity [IDA]
Gene Ontology Cellular Component
- actin cytoskeleton [ISS]
- actin filament [TAS]
- axon [ISS]
- cell junction [IDA]
- cytoplasm [IDA]
- cytoplasmic ribonucleoprotein granule [IDA]
- extracellular vesicular exosome [IDA]
- extrinsic component of cytoplasmic side of plasma membrane [IDA]
- focal adhesion [IDA]
- growth cone [ISS]
- microtubule [IDA]
- microtubule cytoskeleton [ISS]
- midbody [IDA]
- neuron projection [ISS]
- nucleoplasm [IDA]
- plasma membrane [IDA, TAS]
- slit diaphragm [ISS]
Homo sapiens
Co-purification
An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.
Publication
PEX14 is required for microtubule-based peroxisome motility in human cells.
We have established a procedure for isolating native peroxisomal membrane protein complexes from cultured human cells. Protein-A-tagged peroxin 14 (PEX14), a central component of the peroxisomal protein translocation machinery was genomically expressed in Flp-In-293 cells and purified from digitonin-solubilized membranes. Size-exclusion chromatography revealed the existence of distinct multimeric PEX14 assemblies at the peroxisomal membrane. Using mass spectrometric analysis, almost all ... [more]
J. Cell. Sci. May. 15, 2011; 124(0);1759-68 [Pubmed: 21525035]
Throughput
- Low Throughput
Curated By
- BioGRID