BAIT
PEX14
NAPP2, PBD13A, Pex14p, dJ734G22.2
peroxisomal biogenesis factor 14
GO Process (12)
GO Function (6)
GO Component (9)
Gene Ontology Biological Process
- microtubule anchoring [IDA]
- negative regulation of protein binding [IDA]
- negative regulation of protein homotetramerization [IDA]
- negative regulation of sequence-specific DNA binding transcription factor activity [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- peroxisome organization [IGI, ISS]
- peroxisome transport along microtubule [IDA]
- protein complex assembly [IDA]
- protein homooligomerization [IDA]
- protein import into peroxisome matrix [IMP]
- protein import into peroxisome matrix, substrate release [IDA]
- protein import into peroxisome matrix, translocation [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
TUBB2A
CDCBM5, TUBB, TUBB2, dJ40E16.7
tubulin, beta 2A class IIa
GO Process (3)
GO Function (0)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Homo sapiens
Co-purification
An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.
Publication
PEX14 is required for microtubule-based peroxisome motility in human cells.
We have established a procedure for isolating native peroxisomal membrane protein complexes from cultured human cells. Protein-A-tagged peroxin 14 (PEX14), a central component of the peroxisomal protein translocation machinery was genomically expressed in Flp-In-293 cells and purified from digitonin-solubilized membranes. Size-exclusion chromatography revealed the existence of distinct multimeric PEX14 assemblies at the peroxisomal membrane. Using mass spectrometric analysis, almost all ... [more]
J. Cell. Sci. May. 15, 2011; 124(0);1759-68 [Pubmed: 21525035]
Throughput
- Low Throughput
Curated By
- BioGRID