BAIT

GRB2

ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, NCKAP2
growth factor receptor-bound protein 2
Homo sapiens
PREY

PTPRJ

CD148, DEP1, HPTPeta, R-PTP-ETA, SCC1
protein tyrosine phosphatase, receptor type, J
GO Process (17)
GO Function (9)
GO Component (8)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Selected reaction monitoring mass spectrometry reveals the dynamics of signaling through the GRB2 adaptor.

Bisson N, James DA, Ivosev G, Tate SA, Bonner R, Taylor L, Pawson T

Signaling pathways are commonly organized through inducible protein-protein interactions, mediated by adaptor proteins that link activated receptors to cytoplasmic effectors. However, we have little quantitative data regarding the kinetics with which such networks assemble and dissolve to generate specific cellular responses. To address this deficiency, we designed a mass spectrometry method, affinity purification-selected reaction monitoring (AP-SRM), which we used to ... [more]

Nat. Biotechnol. Jul. 01, 2011; 29(7);653-8 [Pubmed: 21706016]

Throughput

  • Low Throughput

Ontology Terms

  • cell line: hek-293t cell (BTO:0002181)

Additional Notes

  • Exogenous expression of bait

Curated By

  • BioGRID