BAIT

TAF3

TAF140, TAFII-140, TAFII140, RP11-379F12.5
TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 140kDa
Homo sapiens
PREY

KPNA2

IPOA1, QIP2, RCH1, SRP1alpha
karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

The nuclear import of TAF10 is regulated by one of its three histone fold domain-containing interaction partners.

Soutoglou E, Demeny MA, Scheer E, Fienga G, Sassone-Corsi P, Tora L

TFIID, comprising the TATA box binding protein (TBP) and 13 TBP-associated factors (TAFs), plays a role in nucleation in the assembly of the RNA polymerase II preinitiation complexes on protein-encoding genes. TAFs are shared among other transcription regulatory complexes (e.g., SAGA, TBP-free TAF-containing complex [TFTC], STAGA, and PCAF/GCN5). Human TAF10, a subunit of both TFIID and TFTC, has three histone ... [more]

Mol. Cell. Biol. May. 01, 2005; 25(10);4092-104 [Pubmed: 15870280]

Throughput

  • Low Throughput

Curated By

  • BioGRID