BAIT

PUM2

PUMH2, PUML2
pumilio RNA-binding family member 2
GO Process (0)
GO Function (2)
GO Component (4)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

DAZL

DAZH, DAZL1, DAZLA, SPGYLA
deleted in azoospermia-like
GO Process (2)
GO Function (3)
GO Component (0)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Human Pumilio-2 is expressed in embryonic stem cells and germ cells and interacts with DAZ (Deleted in AZoospermia) and DAZ-like proteins.

Moore FL, Jaruzelska J, Fox MS, Urano J, Firpo MT, Turek PJ, Dorfman DM, Pera RA

Early in development, a part of the embryo is set aside to become the germ cell lineage that will ultimately differentiate to form sperm and eggs and transmit genetic information to the next generation. Men with deletions encompassing the Y-chromosome DAZ genes have few or no germ cells but are otherwise healthy, indicating they harbor specific defects in formation or ... [more]

Proc. Natl. Acad. Sci. U.S.A. Jan. 21, 2003; 100(2);538-43 [Pubmed: 12511597]

Throughput

  • Low Throughput

Additional Notes

  • figure 6.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
DAZL PUM2
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
2813172

Curated By

  • BioGRID