MARCH5
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
FIS1
Gene Ontology Biological Process
- calcium-mediated signaling using intracellular calcium source [IMP]
- mitochondrial fission [IDA, IMP]
- mitochondrial fragmentation involved in apoptotic process [IDA, IMP]
- mitochondrial fusion [IMP]
- mitochondrion degradation [IDA]
- mitochondrion morphogenesis [IMP]
- negative regulation of endoplasmic reticulum calcium ion concentration [IMP]
- peroxisome fission [IDA, IMP]
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- positive regulation of cytosolic calcium ion concentration [IMP]
- positive regulation of intrinsic apoptotic signaling pathway [IMP]
- positive regulation of mitochondrial calcium ion concentration [IMP]
- positive regulation of mitochondrial fission [IDA]
- positive regulation of protein targeting to membrane [IDA]
- protein homooligomerization [IMP]
- protein targeting to mitochondrion [IMP]
- regulation of mitochondrion organization [IMP]
- release of cytochrome c from mitochondria [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
A novel mitochondrial ubiquitin ligase plays a critical role in mitochondrial dynamics.
In this study, we have identified a novel mitochondrial ubiquitin ligase, designated MITOL, which is localized in the mitochondrial outer membrane. MITOL possesses a Plant Homeo-Domain (PHD) motif responsible for E3 ubiquitin ligase activity and predicted four-transmembrane domains. MITOL displayed a rapid degradation by autoubiquitination activity in a PHD-dependent manner. HeLa cells stably expressing a MITOL mutant lacking ubiquitin ligase ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID