BAIT
NOS2
HEP-NOS, INOS, NOS, NOS2A
nitric oxide synthase 2, inducible
GO Process (21)
GO Function (11)
GO Component (5)
Gene Ontology Biological Process
- arginine catabolic process [IDA]
- blood coagulation [TAS]
- defense response to Gram-negative bacterium [NAS]
- defense response to bacterium [IMP, ISS]
- inflammatory response [IBA]
- innate immune response in mucosa [NAS]
- interaction with host [TAS]
- negative regulation of blood pressure [IBA]
- negative regulation of gene expression [IGI]
- nitric oxide biosynthetic process [IDA]
- nitric oxide mediated signal transduction [IBA]
- peptidyl-cysteine S-nitrosylation [ISS]
- phagosome maturation [TAS]
- positive regulation of guanylate cyclase activity [IBA]
- positive regulation of killing of cells of other organism [IMP]
- positive regulation of leukocyte mediated cytotoxicity [TAS]
- positive regulation of vasodilation [IBA]
- regulation of cellular respiration [TAS]
- regulation of insulin secretion [IMP]
- response to bacterium [NAS]
- superoxide metabolic process [ISS]
Gene Ontology Molecular Function- FMN binding [ISS]
- NADP binding [TAS]
- NADPH-hemoprotein reductase activity [IBA]
- arginine binding [ISS]
- flavin adenine dinucleotide binding [ISS]
- heme binding [ISS]
- nitric-oxide synthase activity [IDA]
- protein binding [IPI]
- protein homodimerization activity [ISS]
- receptor binding [IPI]
- tetrahydrobiopterin binding [ISS]
- FMN binding [ISS]
- NADP binding [TAS]
- NADPH-hemoprotein reductase activity [IBA]
- arginine binding [ISS]
- flavin adenine dinucleotide binding [ISS]
- heme binding [ISS]
- nitric-oxide synthase activity [IDA]
- protein binding [IPI]
- protein homodimerization activity [ISS]
- receptor binding [IPI]
- tetrahydrobiopterin binding [ISS]
Homo sapiens
PREY
HSPD1
CPN60, GROEL, HLD4, HSP-60, HSP60, HSP65, HuCHA60, SPG13
heat shock 60kDa protein 1 (chaperonin)
GO Process (24)
GO Function (11)
GO Component (16)
Gene Ontology Biological Process
- 'de novo' protein folding [ISS]
- ATP catabolic process [ISS]
- B cell activation [IDA]
- B cell cytokine production [IDA]
- B cell proliferation [IDA]
- MyD88-dependent toll-like receptor signaling pathway [IDA]
- T cell activation [IDA]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- chaperone-mediated protein complex assembly [ISS]
- isotype switching to IgG isotypes [IDA]
- negative regulation of apoptotic process [IMP]
- positive regulation of T cell activation [IDA, ISS]
- positive regulation of T cell mediated immune response to tumor cell [IDA]
- positive regulation of apoptotic process [IMP]
- positive regulation of interferon-alpha production [IDA]
- positive regulation of interferon-gamma production [IDA, ISS]
- positive regulation of interleukin-10 production [IDA]
- positive regulation of interleukin-12 production [IDA]
- positive regulation of interleukin-6 production [IDA]
- positive regulation of macrophage activation [IDA]
- protein maturation [ISS]
- protein refolding [IDA]
- protein stabilization [IMP, ISS]
- response to unfolded protein [IDA]
Gene Ontology Molecular Function- ATPase activity [ISS]
- DNA replication origin binding [ISS]
- chaperone binding [IPI]
- double-stranded RNA binding [IDA]
- lipopolysaccharide binding [IDA]
- p53 binding [IPI]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- single-stranded DNA binding [ISS]
- ubiquitin protein ligase binding [IPI]
- unfolded protein binding [IC, ISS]
- ATPase activity [ISS]
- DNA replication origin binding [ISS]
- chaperone binding [IPI]
- double-stranded RNA binding [IDA]
- lipopolysaccharide binding [IDA]
- p53 binding [IPI]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- single-stranded DNA binding [ISS]
- ubiquitin protein ligase binding [IPI]
- unfolded protein binding [IC, ISS]
Gene Ontology Cellular Component
- cell surface [IDA]
- coated pit [IDA]
- coated vesicle [IDA]
- cyclin-dependent protein kinase activating kinase holoenzyme complex [IDA]
- cytoplasm [IDA]
- cytosol [IDA]
- early endosome [IDA]
- extracellular space [IDA]
- extracellular vesicular exosome [IDA]
- lipopolysaccharide receptor complex [IDA]
- membrane [IDA]
- mitochondrial inner membrane [ISS]
- mitochondrial matrix [TAS]
- mitochondrion [IDA]
- protein complex [IDA]
- secretory granule [ISS]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Proteomic analysis of the NOS2 interactome in human airway epithelial cells.
The cytokine-inducible isoform of nitric oxide synthase (NOS2) is constitutively expressed in human respiratory epithelia and is upregulated in inflammatory lung disease. Here, we sought to better define the protein interactions that may be important for NOS2 activity and stability, as well as to identify potential targets of NOS2-derived NO, in the respiratory epithelium. We overexpressed Flag-tagged, catalytically-inactive NOS2 in ... [more]
Nitric Oxide Feb. 21, 2013; 0(0); [Pubmed: 23438482]
Throughput
- High Throughput
Ontology Terms
- cell line: a-549 cell (BTO:0000018) [bronchiolo-alveolar adenocarcinoma (DOID:4926)]
Additional Notes
- exogenous expression of bait
Curated By
- BioGRID