BAIT

NOS2

HEP-NOS, INOS, NOS, NOS2A
nitric oxide synthase 2, inducible
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Proteomic analysis of the NOS2 interactome in human airway epithelial cells.

Foster MW, Thompson JW, Forrester MT, Sha Y, McMahon TJ, Bowles DE, Moseley MA, Marshall HE

The cytokine-inducible isoform of nitric oxide synthase (NOS2) is constitutively expressed in human respiratory epithelia and is upregulated in inflammatory lung disease. Here, we sought to better define the protein interactions that may be important for NOS2 activity and stability, as well as to identify potential targets of NOS2-derived NO, in the respiratory epithelium. We overexpressed Flag-tagged, catalytically-inactive NOS2 in ... [more]

Nitric Oxide Feb. 21, 2013; 0(0); [Pubmed: 23438482]

Throughput

  • High Throughput

Ontology Terms

  • cell line: a-549 cell (BTO:0000018) [bronchiolo-alveolar adenocarcinoma (DOID:4926)]

Additional Notes

  • exogenous expression of bait

Curated By

  • BioGRID