NOS2
Gene Ontology Biological Process
- arginine catabolic process [IDA]
- blood coagulation [TAS]
- defense response to Gram-negative bacterium [NAS]
- defense response to bacterium [IMP, ISS]
- inflammatory response [IBA]
- innate immune response in mucosa [NAS]
- interaction with host [TAS]
- negative regulation of blood pressure [IBA]
- negative regulation of gene expression [IGI]
- nitric oxide biosynthetic process [IDA]
- nitric oxide mediated signal transduction [IBA]
- peptidyl-cysteine S-nitrosylation [ISS]
- phagosome maturation [TAS]
- positive regulation of guanylate cyclase activity [IBA]
- positive regulation of killing of cells of other organism [IMP]
- positive regulation of leukocyte mediated cytotoxicity [TAS]
- positive regulation of vasodilation [IBA]
- regulation of cellular respiration [TAS]
- regulation of insulin secretion [IMP]
- response to bacterium [NAS]
- superoxide metabolic process [ISS]
Gene Ontology Molecular Function- FMN binding [ISS]
- NADP binding [TAS]
- NADPH-hemoprotein reductase activity [IBA]
- arginine binding [ISS]
- flavin adenine dinucleotide binding [ISS]
- heme binding [ISS]
- nitric-oxide synthase activity [IDA]
- protein binding [IPI]
- protein homodimerization activity [ISS]
- receptor binding [IPI]
- tetrahydrobiopterin binding [ISS]
- FMN binding [ISS]
- NADP binding [TAS]
- NADPH-hemoprotein reductase activity [IBA]
- arginine binding [ISS]
- flavin adenine dinucleotide binding [ISS]
- heme binding [ISS]
- nitric-oxide synthase activity [IDA]
- protein binding [IPI]
- protein homodimerization activity [ISS]
- receptor binding [IPI]
- tetrahydrobiopterin binding [ISS]
CUL5
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Proteomic analysis of the NOS2 interactome in human airway epithelial cells.
The cytokine-inducible isoform of nitric oxide synthase (NOS2) is constitutively expressed in human respiratory epithelia and is upregulated in inflammatory lung disease. Here, we sought to better define the protein interactions that may be important for NOS2 activity and stability, as well as to identify potential targets of NOS2-derived NO, in the respiratory epithelium. We overexpressed Flag-tagged, catalytically-inactive NOS2 in ... [more]
Throughput
- High Throughput
Ontology Terms
- cell line: a-549 cell (BTO:0000018) [bronchiolo-alveolar adenocarcinoma (DOID:4926)]
Additional Notes
- exogenous expression of bait
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NOS2 CUL5 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID