BAIT
YWHAZ
1110013I11Rik, 14-3-3zeta, AI596267, AL022924, AU020854
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
GO Process (7)
GO Function (7)
GO Component (11)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
ARHGEF2
AA408978, GEF, GEF-H1, GEFH1, LFP40, Lbcl1, Lfc, P40, mKIAA0651
rho/rac guanine nucleotide exchange factor (GEF) 2
GO Process (14)
GO Function (6)
GO Component (11)
Gene Ontology Biological Process
- actin filament organization [IPI, ISO]
- cell morphogenesis [IDA, ISO]
- cellular response to muramyl dipeptide [ISO]
- establishment of mitotic spindle orientation [IMP]
- negative regulation of microtubule depolymerization [ISO]
- negative regulation of neurogenesis [IMP]
- positive regulation of NF-kappaB transcription factor activity [ISO]
- positive regulation of Rac GTPase activity [ISO]
- positive regulation of Rho GTPase activity [IDA, ISO]
- positive regulation of interleukin-6 production [ISO]
- positive regulation of peptidyl-tyrosine phosphorylation [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of tumor necrosis factor production [ISO]
- regulation of Rho protein signal transduction [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Transgenic mouse proteomics identifies new 14-3-3-associated proteins involved in cytoskeletal rearrangements and cell signaling.
Identification of protein-protein interactions is crucial for unraveling cellular processes and biochemical mechanisms of signal transduction. Here we describe, for the first time, the application of the tandem affinity purification (TAP) and LC-MS method to the characterization of protein complexes from transgenic mice. The TAP strategy developed in transgenic mice allows the emplacement of complexes in their physiological environment in ... [more]
Mol. Cell Proteomics Dec. 01, 2006; 5(12);2211-27 [Pubmed: 16959763]
Throughput
- High Throughput
Curated By
- BioGRID