BAIT
YWHAZ
1110013I11Rik, 14-3-3zeta, AI596267, AL022924, AU020854
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
GO Process (7)
GO Function (7)
GO Component (11)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
ATP2B2
D6Abb2e, Gena300, PMCA2, Tmy, dfw, jog, wms, wri
ATPase, Ca++ transporting, plasma membrane 2
GO Process (31)
GO Function (9)
GO Component (12)
Gene Ontology Biological Process
- ATP catabolic process [IMP]
- auditory receptor cell stereocilium organization [IMP]
- cGMP metabolic process [IMP]
- calcium ion export [ISO]
- calcium ion transmembrane transport [ISO]
- calcium ion transport [IMP, ISO]
- cell morphogenesis [IMP]
- cellular calcium ion homeostasis [IMP]
- cerebellar Purkinje cell differentiation [IMP]
- cerebellar Purkinje cell layer morphogenesis [IMP]
- cerebellar granule cell differentiation [IMP]
- cerebellum development [IMP]
- cochlea development [IMP]
- cytosolic calcium ion homeostasis [IMP, ISO]
- detection of mechanical stimulus involved in sensory perception of sound [IMP]
- inner ear development [IMP]
- inner ear morphogenesis [IMP]
- inner ear receptor cell differentiation [IMP]
- lactation [IMP]
- locomotion [IMP]
- locomotory behavior [IMP]
- neuromuscular process controlling balance [IMP]
- neuron differentiation [ISO]
- organelle organization [IMP]
- otolith mineralization [IMP]
- positive regulation of calcium ion transport [IMP]
- regulation of cell size [IMP]
- regulation of synaptic plasticity [IMP]
- sensory perception of sound [IGI, IMP, ISO]
- serotonin metabolic process [IMP]
- synapse organization [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Transgenic mouse proteomics identifies new 14-3-3-associated proteins involved in cytoskeletal rearrangements and cell signaling.
Identification of protein-protein interactions is crucial for unraveling cellular processes and biochemical mechanisms of signal transduction. Here we describe, for the first time, the application of the tandem affinity purification (TAP) and LC-MS method to the characterization of protein complexes from transgenic mice. The TAP strategy developed in transgenic mice allows the emplacement of complexes in their physiological environment in ... [more]
Mol. Cell Proteomics Dec. 01, 2006; 5(12);2211-27 [Pubmed: 16959763]
Throughput
- High Throughput
Curated By
- BioGRID