BAIT
YWHAZ
1110013I11Rik, 14-3-3zeta, AI596267, AL022924, AU020854
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
GO Process (7)
GO Function (7)
GO Component (11)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
CCDC88A
A430106J12Rik, AI848406, Ape, C130096N06Rik, C330012F17Rik, D130005J21Rik, Girdin, Giv, Grdn, Hkrp1, RP23-92B18.2
coiled coil domain containing 88A
GO Process (10)
GO Function (6)
GO Component (8)
Gene Ontology Biological Process
- TOR signaling [IDA]
- activation of protein kinase B activity [IDA]
- cell migration [ISO]
- lamellipodium assembly [ISO]
- membrane organization [ISO]
- regulation of DNA replication [IMP]
- regulation of actin cytoskeleton organization [ISO]
- regulation of cell proliferation [IMP]
- regulation of neuron projection development [IDA]
- regulation of protein phosphorylation [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Transgenic mouse proteomics identifies new 14-3-3-associated proteins involved in cytoskeletal rearrangements and cell signaling.
Identification of protein-protein interactions is crucial for unraveling cellular processes and biochemical mechanisms of signal transduction. Here we describe, for the first time, the application of the tandem affinity purification (TAP) and LC-MS method to the characterization of protein complexes from transgenic mice. The TAP strategy developed in transgenic mice allows the emplacement of complexes in their physiological environment in ... [more]
Mol. Cell Proteomics Dec. 01, 2006; 5(12);2211-27 [Pubmed: 16959763]
Throughput
- High Throughput
Curated By
- BioGRID