BAIT
YWHAZ
1110013I11Rik, 14-3-3zeta, AI596267, AL022924, AU020854
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
GO Process (7)
GO Function (7)
GO Component (11)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
DMTN
AI325486, Epb4.9, Epb49, dematin
dematin actin binding protein
GO Process (27)
GO Function (4)
GO Component (13)
Gene Ontology Biological Process
- actin cytoskeleton organization [IMP]
- actin filament bundle assembly [ISO]
- actin filament reorganization [IMP]
- calcium-mediated signaling using extracellular calcium source [IMP]
- calcium-mediated signaling using intracellular calcium source [IMP]
- cellular response to cAMP [ISO]
- cellular response to calcium ion [IMP]
- erythrocyte development [IMP]
- negative regulation of cell-substrate adhesion [IMP]
- negative regulation of focal adhesion assembly [IMP]
- negative regulation of peptidyl-serine phosphorylation [IMP]
- negative regulation of peptidyl-threonine phosphorylation [IMP]
- negative regulation of peptidyl-tyrosine phosphorylation [IMP]
- negative regulation of protein targeting to membrane [IMP]
- negative regulation of substrate adhesion-dependent cell spreading [IMP]
- positive regulation of blood coagulation [IMP]
- positive regulation of fibroblast migration [IDA]
- positive regulation of integrin-mediated signaling pathway [IMP]
- positive regulation of platelet aggregation [IMP]
- positive regulation of substrate adhesion-dependent cell spreading [IMP]
- positive regulation of wound healing [IMP]
- protein complex assembly [IDA, ISO]
- protein secretion by platelet [IMP]
- regulation of actin cytoskeleton organization [ISO]
- regulation of cell shape [IDA, IMP, ISO]
- regulation of filopodium assembly [ISO]
- regulation of lamellipodium assembly [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- actin filament [ISO]
- cell [IMP]
- cell projection membrane [IDA]
- cortical cytoskeleton [IDA]
- cytoplasmic membrane-bounded vesicle [ISO]
- cytosol [IDA, ISO]
- intracellular [IMP]
- membrane [IDA]
- neuronal postsynaptic density [IDA]
- perinuclear region of cytoplasm [IDA]
- plasma membrane [ISO]
- platelet dense tubular network membrane [ISO]
- spectrin-associated cytoskeleton [ISO]
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Transgenic mouse proteomics identifies new 14-3-3-associated proteins involved in cytoskeletal rearrangements and cell signaling.
Identification of protein-protein interactions is crucial for unraveling cellular processes and biochemical mechanisms of signal transduction. Here we describe, for the first time, the application of the tandem affinity purification (TAP) and LC-MS method to the characterization of protein complexes from transgenic mice. The TAP strategy developed in transgenic mice allows the emplacement of complexes in their physiological environment in ... [more]
Mol. Cell Proteomics Dec. 01, 2006; 5(12);2211-27 [Pubmed: 16959763]
Throughput
- High Throughput
Curated By
- BioGRID