YWHAZ
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
LDB1
Gene Ontology Biological Process
- Wnt signaling pathway [IMP]
- anterior/posterior axis specification [IMP]
- cellular component assembly [IGI]
- cerebellar Purkinje cell differentiation [IMP]
- cerebellum development [IMP]
- epithelial structure maintenance [IGI]
- gastrulation with mouth forming second [IMP]
- hair follicle development [IGI]
- head development [IGI]
- histone H3-K4 acetylation [IMP]
- negative regulation of erythrocyte differentiation [IMP]
- negative regulation of transcription, DNA-templated [ISO]
- positive regulation of cell adhesion [IGI]
- positive regulation of hemoglobin biosynthetic process [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IGI, IMP, ISO]
- primitive erythrocyte differentiation [TAS]
- regulation of DNA-templated transcription, elongation [IMP]
- regulation of nucleic acid-templated transcription [IPI]
- somatic stem cell maintenance [IGI]
- transcription from RNA polymerase II promoter [IDA]
- transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery [IMP]
Gene Ontology Molecular Function- DNA binding [IDA]
- LIM domain binding [IDA, IPI, ISO]
- RNA polymerase II activating transcription factor binding [ISO]
- chromatin binding [IDA]
- enhancer sequence-specific DNA binding [IDA]
- enzyme binding [IPI, ISO]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- protein self-association [IPI]
- transcription factor binding transcription factor activity [IPI]
- DNA binding [IDA]
- LIM domain binding [IDA, IPI, ISO]
- RNA polymerase II activating transcription factor binding [ISO]
- chromatin binding [IDA]
- enhancer sequence-specific DNA binding [IDA]
- enzyme binding [IPI, ISO]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- protein self-association [IPI]
- transcription factor binding transcription factor activity [IPI]
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Transgenic mouse proteomics identifies new 14-3-3-associated proteins involved in cytoskeletal rearrangements and cell signaling.
Identification of protein-protein interactions is crucial for unraveling cellular processes and biochemical mechanisms of signal transduction. Here we describe, for the first time, the application of the tandem affinity purification (TAP) and LC-MS method to the characterization of protein complexes from transgenic mice. The TAP strategy developed in transgenic mice allows the emplacement of complexes in their physiological environment in ... [more]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| YWHAZ LDB1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID