BAIT
YWHAZ
1110013I11Rik, 14-3-3zeta, AI596267, AL022924, AU020854
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
GO Process (7)
GO Function (7)
GO Component (11)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
PDE1B
Pde1b1
phosphodiesterase 1B, Ca2+-calmodulin dependent
GO Process (11)
GO Function (4)
GO Component (1)
Gene Ontology Biological Process
- cAMP catabolic process [ISO]
- cGMP catabolic process [ISO]
- cellular response to granulocyte macrophage colony-stimulating factor stimulus [ISO]
- cellular response to macrophage colony-stimulating factor stimulus [ISO]
- locomotory behavior [IMP]
- regulation of dopamine metabolic process [IMP]
- regulation of neurotransmitter levels [IMP]
- response to amphetamine [IGI, IMP]
- serotonin metabolic process [IMP]
- signal transduction [IMP]
- visual learning [IGI, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Transgenic mouse proteomics identifies new 14-3-3-associated proteins involved in cytoskeletal rearrangements and cell signaling.
Identification of protein-protein interactions is crucial for unraveling cellular processes and biochemical mechanisms of signal transduction. Here we describe, for the first time, the application of the tandem affinity purification (TAP) and LC-MS method to the characterization of protein complexes from transgenic mice. The TAP strategy developed in transgenic mice allows the emplacement of complexes in their physiological environment in ... [more]
Mol. Cell Proteomics Dec. 01, 2006; 5(12);2211-27 [Pubmed: 16959763]
Throughput
- High Throughput
Curated By
- BioGRID