BAIT
YWHAZ
1110013I11Rik, 14-3-3zeta, AI596267, AL022924, AU020854
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
GO Process (7)
GO Function (7)
GO Component (11)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
PDE4D
9630011N22Rik, Dpde3, RP24-383A20.1
phosphodiesterase 4D, cAMP specific
GO Process (16)
GO Function (4)
GO Component (2)
Gene Ontology Biological Process
- aging [IMP]
- cAMP catabolic process [IDA, IMP]
- cellular response to epinephrine stimulus [IMP]
- cellular response to lipopolysaccharide [IMP]
- leukocyte migration [IMP]
- multicellular organism growth [IMP]
- negative regulation of heart contraction [IMP]
- negative regulation of peptidyl-serine phosphorylation [IMP]
- negative regulation of relaxation of cardiac muscle [IMP]
- neutrophil chemotaxis [IMP]
- regulation of cardiac muscle cell contraction [IMP]
- regulation of cell communication by electrical coupling involved in cardiac conduction [IC]
- regulation of heart rate [IMP]
- regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum [IMP]
- regulation of ryanodine-sensitive calcium-release channel activity [IMP]
- smooth muscle contraction [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Transgenic mouse proteomics identifies new 14-3-3-associated proteins involved in cytoskeletal rearrangements and cell signaling.
Identification of protein-protein interactions is crucial for unraveling cellular processes and biochemical mechanisms of signal transduction. Here we describe, for the first time, the application of the tandem affinity purification (TAP) and LC-MS method to the characterization of protein complexes from transgenic mice. The TAP strategy developed in transgenic mice allows the emplacement of complexes in their physiological environment in ... [more]
Mol. Cell Proteomics Dec. 01, 2006; 5(12);2211-27 [Pubmed: 16959763]
Throughput
- High Throughput
Curated By
- BioGRID