An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Transgenic mouse proteomics identifies new 14-3-3-associated proteins involved in cytoskeletal rearrangements and cell signaling.

Angrand PO, Segura I, Voelkel P, Ghidelli S, Terry R, Brajenovic M, Vintersten K, Klein R, Superti-Furga G, Drewes G, Kuster B, Bouwmeester T, Acker-Palmer A

Identification of protein-protein interactions is crucial for unraveling cellular processes and biochemical mechanisms of signal transduction. Here we describe, for the first time, the application of the tandem affinity purification (TAP) and LC-MS method to the characterization of protein complexes from transgenic mice. The TAP strategy developed in transgenic mice allows the emplacement of complexes in their physiological environment in ... [more]

Mol. Cell Proteomics Dec. 01, 2006; 5(12);2211-27 [Pubmed: 16959763]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

YWHAZ

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [ISO]poly(A) RNA binding [ISO]protein binding [IPI]protein complex binding [ISO]protein domain specific binding [IDA]protein kinase binding [ISO]transcription factor binding [ISO]

Gene Ontology Cellular Component

PPFIA3