BAIT
YWHAZ
1110013I11Rik, 14-3-3zeta, AI596267, AL022924, AU020854
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
GO Process (7)
GO Function (7)
GO Component (11)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
SLC1A3
AI504299, B430115D02Rik, Eaat1, GLAST, GLAST-1, GLU-T, GluT-1, Gmt1, MGluT1
solute carrier family 1 (glial high affinity glutamate transporter), member 3
GO Process (21)
GO Function (5)
GO Component (11)
Gene Ontology Biological Process
- D-aspartate import [ISO]
- L-glutamate import [IMP, ISO]
- L-glutamate transmembrane transport [IMP, ISO]
- L-glutamate transport [IDA, ISO]
- auditory behavior [IMP]
- cell morphogenesis involved in neuron differentiation [IMP]
- cranial nerve development [IMP]
- gamma-aminobutyric acid biosynthetic process [IMP]
- glutamate biosynthetic process [IMP]
- glutamate metabolic process [IMP]
- malate-aspartate shuttle [ISO]
- neuromuscular process controlling balance [IMP]
- positive regulation of synaptic transmission [IMP]
- response to amino acid [ISO]
- response to antibiotic [IMP]
- response to drug [IMP]
- response to inorganic substance [ISO]
- response to light stimulus [IMP]
- response to wounding [IMP]
- sensory perception of sound [IMP]
- sodium ion transmembrane transport [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Transgenic mouse proteomics identifies new 14-3-3-associated proteins involved in cytoskeletal rearrangements and cell signaling.
Identification of protein-protein interactions is crucial for unraveling cellular processes and biochemical mechanisms of signal transduction. Here we describe, for the first time, the application of the tandem affinity purification (TAP) and LC-MS method to the characterization of protein complexes from transgenic mice. The TAP strategy developed in transgenic mice allows the emplacement of complexes in their physiological environment in ... [more]
Mol. Cell Proteomics Dec. 01, 2006; 5(12);2211-27 [Pubmed: 16959763]
Throughput
- High Throughput
Curated By
- BioGRID