YWHAH
Gene Ontology Biological Process
- cytoskeleton organization [NAS]
- glucocorticoid catabolic process [ISO]
- glucocorticoid receptor signaling pathway [ISO]
- intracellular protein transport [IDA]
- membrane depolarization during action potential [ISO, ISS]
- negative regulation of apoptotic process [NAS]
- negative regulation of dendrite morphogenesis [IDA]
- positive regulation of transcription, DNA-templated [ISO]
- regulation of mitosis [NAS]
- regulation of sodium ion transmembrane transporter activity [ISO, ISS]
- regulation of sodium ion transport [ISO, ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
MARK2
Gene Ontology Biological Process
- establishment of cell polarity [ISO]
- establishment or maintenance of epithelial cell apical/basal polarity [ISO]
- intracellular signal transduction [ISO]
- neuron migration [ISO]
- positive regulation of neuron projection development [ISO]
- protein autophosphorylation [ISO]
- protein phosphorylation [ISO]
- regulation of axonogenesis [ISO]
- regulation of cytoskeleton organization [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Transgenic mouse proteomics identifies new 14-3-3-associated proteins involved in cytoskeletal rearrangements and cell signaling.
Identification of protein-protein interactions is crucial for unraveling cellular processes and biochemical mechanisms of signal transduction. Here we describe, for the first time, the application of the tandem affinity purification (TAP) and LC-MS method to the characterization of protein complexes from transgenic mice. The TAP strategy developed in transgenic mice allows the emplacement of complexes in their physiological environment in ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| MARK2 YWHAH | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| YWHAH MARK2 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.933 | BioGRID | 2670656 |
Curated By
- BioGRID