BAIT

ARHGEF6

1600028C08Rik, 1700038J06Rik, 4930592P22Rik, alpha-PIX, RP23-372E6.1
Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus
PREY

GIT1

Cat-1, p95Cat, RP23-81E16.3
G protein-coupled receptor kinase-interactor 1
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Transgenic mouse proteomics identifies new 14-3-3-associated proteins involved in cytoskeletal rearrangements and cell signaling.

Angrand PO, Segura I, Voelkel P, Ghidelli S, Terry R, Brajenovic M, Vintersten K, Klein R, Superti-Furga G, Drewes G, Kuster B, Bouwmeester T, Acker-Palmer A

Identification of protein-protein interactions is crucial for unraveling cellular processes and biochemical mechanisms of signal transduction. Here we describe, for the first time, the application of the tandem affinity purification (TAP) and LC-MS method to the characterization of protein complexes from transgenic mice. The TAP strategy developed in transgenic mice allows the emplacement of complexes in their physiological environment in ... [more]

Mol. Cell Proteomics Dec. 01, 2006; 5(12);2211-27 [Pubmed: 16959763]

Throughput

  • Low Throughput

Curated By

  • BioGRID