ARHGEF7
Gene Ontology Biological Process
- astrocyte cell migration [ISO]
- focal adhesion assembly [ISO]
- hematopoietic progenitor cell differentiation [IGI]
- lamellipodium assembly [IMP]
- positive regulation of GTPase activity [ISO]
- positive regulation of Rac GTPase activity [IMP, ISO]
- positive regulation of apoptotic process [ISO]
- positive regulation of fibroblast migration [ISO]
- positive regulation of lamellipodium morphogenesis [ISO]
- positive regulation of substrate adhesion-dependent cell spreading [ISO]
- small GTPase mediated signal transduction [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GIT1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Transgenic mouse proteomics identifies new 14-3-3-associated proteins involved in cytoskeletal rearrangements and cell signaling.
Identification of protein-protein interactions is crucial for unraveling cellular processes and biochemical mechanisms of signal transduction. Here we describe, for the first time, the application of the tandem affinity purification (TAP) and LC-MS method to the characterization of protein complexes from transgenic mice. The TAP strategy developed in transgenic mice allows the emplacement of complexes in their physiological environment in ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GIT1 ARHGEF7 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.9465 | BioGRID | 2666881 |
Curated By
- BioGRID