Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

gp25L/emp24/p24 protein family members of the cis-Golgi network bind both COP I and II coatomer.

Dominguez M, Dejgaard K, Fuellekrug J, Dahan S, Fazel A, Paccaud JP, Thomas DY, Bergeron JJ, Nilsson T

Abstract. Five mammalian members of the gp25L/ emp24/p24 family have been identified as major constituents of the cis-Golgi network of rat liver and HeLa cells. Two of these were also found in membranes of higher density (corresponding to the ER), and this correlated with their ability to bind COP I in vitro. This binding was mediated by a K(X)KXX-like retrieval ... [more]

J. Cell Biol. Feb. 23, 1998; 140(4);751-65 [Pubmed: 9472029]

Throughput

  • Low Throughput

Curated By

  • BioGRID