AGO1
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- Notch signaling pathway [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- gene expression [TAS]
- innate immune response [TAS]
- negative regulation of translation involved in gene silencing by miRNA [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- nuclear-transcribed mRNA catabolic process [IDA]
- phosphatidylinositol-mediated signaling [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
DICER1
Gene Ontology Biological Process
- RNA phosphodiester bond hydrolysis [IDA]
- RNA phosphodiester bond hydrolysis, endonucleolytic [IDA, IMP]
- conversion of ds siRNA to ss siRNA [IMP]
- gene expression [TAS]
- negative regulation of Schwann cell proliferation [ISS]
- negative regulation of transcription from RNA polymerase II promoter [ISS]
- nerve development [ISS]
- neuron projection morphogenesis [ISS]
- peripheral nervous system myelin formation [ISS]
- positive regulation of Schwann cell differentiation [ISS]
- positive regulation of myelination [ISS]
- pre-miRNA processing [IDA]
- production of miRNAs involved in gene silencing by miRNA [ISS]
- production of siRNA involved in RNA interference [IDA]
- targeting of mRNA for destruction involved in RNA interference [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mammalian miRNA RISC recruits CAF1 and PABP to affect PABP-dependent deadenylation.
MicroRNAs (miRNAs) inhibit mRNA expression in general by base pairing to the 3'UTR of target mRNAs and consequently inhibiting translation and/or initiating poly(A) tail deadenylation and mRNA destabilization. Here we examine the mechanism and kinetics of miRNA-mediated deadenylation in mouse Krebs-2 ascites extract. We demonstrate that miRNA-mediated mRNA deadenylation occurs subsequent to initial translational inhibition, indicating a two-step mechanism of ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| AGO1 DICER1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9999 | BioGRID | 3097220 | |
| DICER1 AGO1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| AGO1 DICER1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| AGO1 DICER1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | High | - | BioGRID | - | |
| DICER1 AGO1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID