Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Proteomic profiling of the TRAF3 interactome network reveals a new role for the ER-to-Golgi transport compartments in innate immunity.

van Zuylen WJ, Doyon P, Clement JF, Khan KA, D'Ambrosio LM, Do F, St-Amant-Verret M, Wissanji T, Emery G, Gingras AC, Meloche S, Servant MJ

Tumor Necrosis Factor receptor-associated factor-3 (TRAF3) is a central mediator important for inducing type I interferon (IFN) production in response to intracellular double-stranded RNA (dsRNA). Here, we report the identification of Sec16A and p115, two proteins of the ER-to-Golgi vesicular transport system, as novel components of the TRAF3 interactome network. Notably, in non-infected cells, TRAF3 was found associated with markers ... [more]

PLoS Pathog. Jul. 14, 2012; 8(7);e1002747 [Pubmed: 22792062]

Throughput

  • High Throughput

Additional Notes

  • figure S1A.

Curated By

  • BioGRID