GRM5
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SIAH1
Gene Ontology Biological Process
- anatomical structure morphogenesis [TAS]
- apoptotic process [TAS]
- axon guidance [TAS]
- nervous system development [TAS]
- neuron apoptotic process [ISS]
- positive regulation of apoptotic process [IDA]
- positive regulation of intrinsic apoptotic signaling pathway [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [ISS]
- protein catabolic process [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [ISS]
- ubiquitin-dependent protein catabolic process [IDA]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
PKC phosphorylation regulates mGluR5 trafficking by enhancing binding of Siah-1A.
Glutamate is the major excitatory neurotransmitter in the mammalian CNS and acts on both ionotropic and metabotropic glutamate receptors (mGluRs). The mGluRs are widely distributed in the CNS and modulate a variety of neuronal processes, including neurotransmitter release and ion channel function. In hippocampus and cortex, mGluR5 is highly expressed and plays an important role in the regulation of synaptic ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GRM5 SIAH1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID