An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Three binding sites in protein-disulfide isomerase cooperate in collagen prolyl 4-hydroxylase tetramer assembly.

Koivunen P, Salo KE, Myllyharju J, Ruddock LW

Protein-disulfide isomerase (PDI) is a modular polypeptide consisting of four domains, a, b, b', and a'. It is a ubiquitous protein folding catalyst that in addition functions as the beta-subunit in vertebrate collagen prolyl 4-hydroxylase (C-P4H) alpha(2)beta(2) tetramers. We report here that point mutations in the primary peptide substrate binding site in the b' domain of PDI did not inhibit ... [more]

J. Biol. Chem. Feb. 18, 2005; 280(7);5227-35 [Pubmed: 15590633]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

PDIA2

Gene Ontology Biological Process

Gene Ontology Molecular Function

peptidyl-proline 4-dioxygenase activity [IBA]protein binding [IPI]protein disulfide isomerase activity [IBA]

Gene Ontology Cellular Component

SST