BAIT

IML3

MCM19, L000004778, YBR107C
Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; forms a stable complex with Chl4p; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1
Saccharomyces cerevisiae (S288c)
PREY

TUB1

alpha-tubulin TUB1, L000002387, YML085C
Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Iml3p, a component of the Ctf19 complex of the budding yeast kinetochore is required to maintain kinetochore integrity under conditions of spindle stress.

Lahiri S, Mehta GD, Ghosh SK

The Ctf19 multi-protein complex of the central kinetochore in Saccharomyces cerevisiae is required for precise chromosome segregation during mitosis. Out of eleven proteins of this complex, at least six are required for cell survival when microtubules are defective. In order to find individual roles of these proteins in kinetochore stability, double deletion mutants of the corresponding genes were constructed in ... [more]

FEMS Yeast Res. Mar. 10, 2013; 0(0); [Pubmed: 23480593]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: cell cycle progression through the g2/m phase transition (APO:0000257)
  • phenotype: chromosome/plasmid maintenance (APO:0000143)

Additional Notes

  • iml3 chl4 tub1-1 triple mutant displays enhanced minichromosome instability and increased numbers of large-budded arrested cells, relative to the respective double mutants

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
IML3 TUB1
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Low-BioGRID
836428
TUB1 IML3
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Low-BioGRID
836432
IML3 TUB1
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
158248

Curated By

  • BioGRID