BAIT
UBL4A
DX254E, DXS254E, G6PD, GDX, GET5, MDY2, TMA24, UBL4, XX-FW89031B12.1
ubiquitin-like 4A
GO Process (2)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
RAB13
GIG4
RAB13, member RAS oncogene family
GO Process (15)
GO Function (4)
GO Component (14)
Gene Ontology Biological Process
- GTP catabolic process [IBA]
- Rab protein signal transduction [IBA]
- cellular response to insulin stimulus [ISS]
- cortical actin cytoskeleton organization [IBA, ISS]
- endocytic recycling [IMP]
- endosomal transport [IMP]
- endothelial cell chemotaxis [ISS]
- establishment of Sertoli cell barrier [ISS]
- establishment of protein localization to plasma membrane [IMP]
- intracellular protein transport [IBA]
- membrane organization [TAS]
- neuron projection development [IMP]
- protein kinase A signaling [IMP]
- tight junction assembly [IMP]
- trans-Golgi network to recycling endosome transport [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- cytoplasm [IDA]
- cytoplasmic vesicle [IDA]
- cytoplasmic vesicle membrane [TAS]
- endocytic vesicle [IDA]
- extracellular vesicular exosome [IDA]
- insulin-responsive compartment [ISS]
- lamellipodium [ISS]
- lateral plasma membrane [IDA]
- neuron projection [IDA]
- plasma membrane [IDA]
- recycling endosome [IDA]
- tight junction [IDA]
- trans-Golgi network [IDA]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
SGTA Recognizes a Noncanonical Ubiquitin-like Domain in the Bag6-Ubl4A-Trc35 Complex to Promote Endoplasmic Reticulum-Associated Degradation.
Elimination of aberrantly folded polypeptides from the endoplasmic reticulum (ER) by the ER-associated degradation (ERAD) system promotes cell survival under stress conditions. This quality control mechanism requires movement of misfolded proteins across the ER membrane for targeting to the cytosolic proteasome, a process facilitated by a "holdase" complex, consisting of Bag6 and the cofactors Ubl4A and Trc35. This multiprotein complex ... [more]
Cell Rep Dec. 27, 2012; 2(6);1633-44 [Pubmed: 23246001]
Throughput
- High Throughput
Ontology Terms
- cell line: hek-293 cell (BTO:0000007)
Additional Notes
- Flag-tagged Ubl4a together with His-Bag6 is used to pull down interacting proteins which are identified by MS
Curated By
- BioGRID